| 胃癌组织中Bmi-1基因表达的研究 |
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| 引用本文： | 刘嵬,朱斌,刘卫,章福彬,唐郡.胃癌组织中Bmi-1基因表达的研究[J].现代生物医学进展,2011,11(15):2893-2895. |
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| 作者姓名： | 刘嵬 朱斌 刘卫 章福彬 唐郡 |
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| 作者单位： | 解放军第105医院消化内科,安徽合肥,230031 |
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| 摘 要： | 目的:多梳基因家族的Bmi-1被认为是一种癌基因,在多种肿瘤组织中均有表达。本研究主要是检测Bmi-1基因在胃癌组织中的表达及探讨其临床意义。方法:应用RT-PCR和Western blotting方法检测45例胃癌中Bmi-1基因的表达情况,并结合患者的临床病理资料分析与其相关性。结果:Bmi-1基因阳性表达率在胃癌中为88.9%(40/45),癌旁组织为17.7%(8/45),差异有显著性(P<0.05);Bmi-1基因阳性表达率与患者年龄、性别、肿瘤大小及有无淋巴结转移无关,与肿瘤分化程度及TNM分期有关(P<0.05)结论:Bmi-1基因在胃癌中高表达,与胃癌的疾病进展密切相关,检测Bmi-1的表达可作为胃癌生物学行为的一项评估指标。
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| 关 键 词： | Bmi-1基因 胃癌 逆转录-聚合酶链反应 Western免疫印记分析 |
Study on Expression of Bmi-1 in Gastric Carcinoma |
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| LIU Wei,ZHU Bin,LIU Wei,ZHANG Fu-bin,TANG Jun.Study on Expression of Bmi-1 in Gastric Carcinoma[J].Progress in Modern Biomedicine,2011,11(15):2893-2895. |
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| Authors: | LIU Wei ZHU Bin LIU Wei ZHANG Fu-bin TANG Jun |
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| Institution: | LIU Wei,ZHU Bin,ZHANG Fu-bin,TANG Jun (Department of Gastroenterology,the 105th Hospital of PLA,Hefei 230031,China) |
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| Abstract: | Objective:To investigate the expression of Bmi-1 in gastric carcinoma and its significance.BMI-1,a member of the Polycomb Group(PcG) family genes,was a human proto-oncogene.Methods:The expression of Bmi-1 in 45 cases of gastric carcinoma specimens were examined by RT- PCR and Western blotting.The correlation of Bmi-1 expression to clinicopathologic features of gastric carcinoma was analyzed.Results:The positive incidence of Bmi-1 expression in gastric carcinoma was 88.9%,while in its corresponding adjacent ... |
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| Keywords: | Bmi-1 Gastric carcinoma RT- PCR Western blotting |
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