N
6-Substituted AMPs Inhibit Mammalian Deoxynucleotide N-Hydrolase DNPH1 |
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| Authors: | Claire Amiable Sylvie Pochet André Padilla Gilles Labesse Pierre Alexandre Kaminski |
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| Institution: | 1. Institut Pasteur, Unité de Chimie et Biocatalyse, Paris, France.; 2. CNRS, UMR3523, Paris, France.; 3. Université Paris Descartes Sorbonne Paris Cité, Paris, France.; 4. CNRS, UMR5048, Centre de Biochimie Structurale, Montpellier, France.; 5. INSERM, U554, Montpellier, France.; 6. Université Montpellier 1 et 2; IFR3, Montpellier, France .; Rutgers University -New Jersey Medical School, United States of America, |
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| Abstract: | The gene dnph1 (or rcl) encodes a hydrolase that cleaves the 2’-deoxyribonucleoside 5’-monophosphate (dNMP) N-glycosidic bond to yield a free nucleobase and 2-deoxyribose 5-phosphate. Recently, the crystal structure of rat DNPH1, a potential target for anti-cancer therapies, suggested that various analogs of AMP may inhibit this enzyme. From this result, we asked whether N
6-substituted AMPs, and among them, cytotoxic cytokinin riboside 5’-monophosphates, may inhibit DNPH1. Here, we characterized the structural and thermodynamic aspects of the interactions of these various analogs with DNPH1. Our results indicate that DNPH1 is inhibited by cytotoxic cytokinins at concentrations that inhibit cell growth. |
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