| 七叶皂苷对P-糖蛋白功能的影响 |
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| 引用本文： | 刘丽娟,胡东莉,李汇娟,范兰,周宏灏.七叶皂苷对P-糖蛋白功能的影响[J].现代生物医学进展,2010,10(7). |
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| 作者姓名： | 刘丽娟 胡东莉 李汇娟 范兰 周宏灏 |
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| 作者单位： | 中南大学临床药理研究所; |
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| 摘 要： | 目的:探讨七叶皂苷时P-糖蛋白功能的影响.方法:构建稳定表达P-糖蛋白的LLC-PK1细胞系,以real-time RT-PCR和Western Blotting分析P-糖蛋白基因mRNA和蛋白表达,共聚焦显微镜观察P-糖蛋白细胞定位,流式细胞术检测细胞内罗丹明123荧光强度.结果:(1)P-糖蛋白在LLC-PK1细胞中稳定高表达;(2)转染细胞中P-糖蛋白定位在细胞膜上;(3)七叶皂苷抑制P-糖蛋白功能.细胞内罗丹明123荧光强度增加123%,但其抑制效果是维拉帕米的30%.结论:七叶皂苷抑制P-糖蛋白功能,但其抑制效果弱于维拉帕米.
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| 关 键 词： | 七叶皂苷 P-糖蛋白 罗丹明123 |
Effect of aescin on P-glycoprotein activity |
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| LIU Li-juan,HU Dong-li,LI Hui-juan,ZHOU Hong-hao.Effect of aescin on P-glycoprotein activity[J].Progress in Modern Biomedicine,2010,10(7). |
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| Authors: | LIU Li-juan HU Dong-li LI Hui-juan ZHOU Hong-hao |
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| Institution: | LIU Li-juan,HU Dong-li,LI Hui-juan,ZHOU Hong-hao(Institute of Clinical Pharmacology,Central South University,Changsha 410078,Hunan,China) |
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| Abstract: | Objective:This study was to investigate the effect of aescin on P-glycoprotein(P-gp) activity.Methods:MDR1 was transfected into LLC-PK1 cells.Expressions of mRNA and protein of MDR1 were measured by real-rime RT-PCR and Western Blotting,respectively,localization of P-gp by confocal microscopy.The fluorescence intensity of Rho123 was assayed by flow cytometry(FCM).Results:(1) Stable transfection MDR1 could increase the P-gp expression in RNA and protein level;(2) P-gp located in the membrance of LLC-PK1 cell... |
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| Keywords: | Aescin P-glycopretein Rho123 |
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