Studies on Tn951 (lac+) expression in Agrobacterium |
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Authors: | P A Borland and G R K Sastry |
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Institution: | (1) Department of Genetics, University of Leeds, LS2 9JT Leeds, England |
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Abstract: | Summary None of the Agrobacterium tumefaciens and A. rubi strains tested produces detectable amounts of -galactosidase although they are capable of utilizing lactose as sole source of carbon. This opportunity was taken to investigate the expression of lac transposon Tn951 (Cornelis et al. 1978) in Agrobacterium with the ultimate goal of using this system to investigate alien gene expression. When the transposon was introduced with the help of a broad-host range plasmid, RP1, the transconjugants produced significant quantities of -galactosidase which was inducible by isopropyl--D-thiogalactopyranoside. Tn951 was capable of restoring the Lac+ phenotype to an A. tumefaciens mutant not capable of using lactose. Cellobiose, a known inducer of aldohexopyranoside: cytochrome c oxidoreductase which regulates the characteristic 3-ketolactose production in Agrobacterium: van Beeumen and De Ley (1968), had no effect on -galactosidase activity.Abbreviations NCPPB
National Collection of Plant Pathogenic Bacteria, Harpenden
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km
kanamycin resistance
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str
streptomycin resistance
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rif
r
rifampicin resistance |
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