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Isolation of living megaspores ofTorenia fournieri
Authors:Z Kristóf  K Imre
Institution:(1) Department of Plant Anatomy, Eötvös L. University, Puskin u. 11–13, H-1088 Budapest, Hungary
Abstract:Summary Viable megaspore protoplasts ofTorenia fournieri were isolated with enzymatic digestion and mild osmotic shock. With this method, living haploid protoplasts were released from the ovules, while very few sporophytic protoplasts were produced.
Keywords:Megaspore  Embryo sac  Protoplast  Torenia
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