小鼠胸腺基质细胞系分泌的趋化因子的分析

利用Boyden小室法和FACS分析法,我们分析了五株小鼠胸腺基质细胞系(MTSC)培养上清液对中性粒细胞,单核巨噬细胞和淋巴细胞的化学趋化因子(Chemokines)活性,及定向迁移的淋巴细胞中B细胞、CD 4~ CD 8~-和CD4~-CD8~ T细胞的比例。结果显示,五株MTSC的培养上清液对上述靶细胞均有不同程度的趋化作用.MTSC细胞分泌趋化因子的情况可分为三类:1.MTEC 1和MTEC 2产生的Chemokine(s)对中性粒细胞和淋巴细胞的趋化作用相对较强;2.MTDC 4分泌的Chemokine(s)主要作用于单核巨噬细胞;3.MTEC 3和MTEC 5分泌的Chemokine(s)对多种类型的靶细胞,包括中性粒细胞、单核巨噬细胞和淋巴细胞,表现的趋化作用没有明显的强弱之分。MTSC-SN对B细胞的趋化活性普遍高于对T细胞的趋化活性,对CD 4~-CK 8~ T细胞的趋化活性高于对CD 4~ CD 8~-T细胞的趋化活性。MTSC-SN中趋化因子的分析,有利于新型chemokines的发现及其生物功能的阐明,并可进一步研究Chemok-ine(s)在T细胞发育中的作用。

ANALYSIS OF CHEMOKINE (S) PRODUCED BY MOUSE THYMIC STROMAL CELL LINES

By using Boyden Chamber and PACS methods, we analysed the chemotactic activity of five mouse thymic stromal cell (MTSC) lines' culture supernatant for nautrophil, monocyte/macrophage and lymphocyte, and the phenotype of the attracted lymphocytes. MTEC1, MTEC 2. MTEC 3, MTEC 5 are mouse thymus epithelial cell lines. MTDC 4 is a mouse thymus dendritic cell line. The results indicate that all of the five MTSC super-natants have chemotactic activity for the target cells mentioned above but in different degree. Chemokines produced by the MTSC cells can be classified into three categories: (1) Chemokines produced by MTEC 1 and MTEC 2 are more potentfor the attraction of neutrophils and lymphocytes. (2) MTDC 4 derived chemokines are highly potent for the attraction of monpcytes/macrophoges. (3) Chemokines produced by MTEC 3 and MTEC 5 are equally attractive to neutrophils, lymphocytes and monocytes/macrophages. Most of the MTSC-SN are more potent for the migration of B lymphocyte than for T lymphocyte. The MTSC-SN exhibits higher chemotactic activity to CD 4~CD 8 T cell subset than to CD4 CD8- T cell subset. The characterization of chemokines in MTSC-SN will benefit the finding of new chemokines and the analysis of the mechanism of thymus homing.