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Detection and cloning of expressed sequences linked to a target gene |
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| Authors: | C. E. Vallejos J. J. Malandro K. Sheehy M. J. Zimmermann |
| Affiliation: | (1) Department of Horticultural Sciences, and Plant Molecular and Cellular Biology Graduate Program, University of Florida, 1143 Fifield Hall, Gainesville, FL 32611, USA e-mail: Vallejos@ufl.edu, Fax: +1 353 392-6479, US;(2) Centro Nacional de Pesquisa de Arroz e Feijao, Empresa Brasileira de Pesquisa Agropecuaria, Goiania, GO, Brazil, Present address: FAO-SDRR, Office: C-692, Via delle Terme di Caracalla, 00100-Rome, Italy, BR |
| Abstract: | DNA markers tightly linked to a target gene are essential starting points for positional cloning. We combined ”differential display of mRNA” and ”bulked segregant analysis” in order to detect and clone ten expressed sequences as markers linked to a virus resistance gene in Phaseolus vulgaris. The combination of these two procedures could be used in lieu of positional cloning, provided polymorphisms detectable by differential display exist in the target gene. Isolation of expressed sequences from specific chromosome regions can also be accomplished by combining these procedures. Received: 8 July 1999 / Accepted: 21 March 2000 |
| Keywords: | Differential display of mRNA Bulked segregant analysis Bean common mosaic virus Linkage analysis |
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