Proteomic analysis of cold stress-responsive proteins in <Emphasis Type="Italic">Thellungiella</Emphasis> rosette leaves |
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Authors: | Fei Gao Yijun Zhou Weiping Zhu Xiaofeng Li Liumin Fan Genfa Zhang |
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Institution: | (1) Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, 100875 Beijing, China;(2) College of Life and Environmental Sciences, Minzu University of China, 100081 Beijing, China;(3) Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, 100871 Beijing, China; |
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Abstract: | Low temperature is one of the most severe environmental factors that impair plant growth and agricultural production. To investigate
how Thellungiella halophila, an Arabidopsis-like extremophile, adapts to cold stress, a comparative proteomic approach based on two-dimensional electrophoresis was adopted
to identify proteins that changed in abundance in Thellungiella rosette leaves during short term (6 h, 2 and 5 days) and long term (24 days) exposure to cold stress. Sixty-six protein spots
exhibited significant change at least at one time point and maximal cold stress induced-proteome change was found in long-term
cold stress group while the minimal change was found in 6-h cold treatment group. Fifty protein spots were identified by mass
spectrometry analysis. The identified proteins mainly participate in photosynthesis, RNA metabolism, defense response, energy
pathway, protein synthesis, folding and degradation, cell wall and cytoskeleton and signal transduction. These proteins might
work cooperatively to establish a new homeostasis under cold stress. Nearly half of the identified cold-responsive proteins
were associated with various aspects of chloroplast physiology suggesting that the cold stress tolerance of T. halophila is achieved, at least partly, by regulation of chloroplast function. All protein spots involved in RNA metabolism, defense
response, protein synthesis, folding and degradation were found to be upregulated markedly by cold treatment, indicating enhanced
RNA metabolism, defense and protein metabolism may play crucial roles in cold tolerance mechanism in T. halophila. |
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