Structure of mycoside F, a family of trehalose-containing glycolipids of Mycobacterium fortuitum |
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Authors: | Nicolas Gautier Luz María López Marín Marie-Antoinette Lanéelle Mamadou Daffé |
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Institution: | Département des Glycoconjugués et Biomembranes LPTF du CNRS, Toulouse, France. |
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Abstract: | Nuclear magnetic resonance spectroscopy, fast-atom bombardment mass spectrometry, gas chromatography-mass spectrometry, as well as chemical degradations were used to elucidate the structure of the major glycolipids of Mycobacterium fortuitum. Three main glycoconjugates were detected and their structures established as 2,3-diacyl, 2,3,4- and 2,3,6-triacyl trehalose. The characteristic infrared spectrum which led to their original designation as mycoside F, a family of glycolipids limited in distribution to M. fortuitum, was due to the nature of the fatty acyl substiuents identified primarily as 2-methyl-octadecen-2-oyl. The antigenic glycolipids typified the biovar. fortuitum, thus allowing its easy recognition from the C-mycoside glycopeptidolipid-containing biovar. peregrinum. |
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Keywords: | Mycobacterium fortuitum Antigen Glycolipid Acyl-trehalose Mycoside F |
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