An optimized culture medium for human vascular endothelial cells from umbilical cord veins |
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Authors: | Peter Friedl Dieter Tatje Rüdiger Czpla |
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Institution: | (1) GBF-Gesellschaft für Biotechnologische Forschung mbH., Mascheroder Weg 1, D-3000 Braunschweig, FRG |
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Abstract: | Various polypeptide growth factors, culture substrates, basal media, sera and further supplements were assayed for improvement of growth of human vascular endothelial cells from umbilical cord veins. The resulting optimized medium consisted of gelatinized culture substrates, a mixture (1:1) of Iscove's MDM and Ham's F12 basal media supplemented with 20% newborn calf serum, 500 ng/ml crude fibroblast growth factor, 20 ng/ml epidermal growth factor, 5 g/ml transferrin, 5 g/ml insulin and 10 g/ml heparin. The medium allowed long term cultivation of HUVEC up to 45 generations with maximal cell densities of about 105 cells per cm2 and a minimal doubling time of about 14 hours at low cell densities.Abbreviations HUVEC
Human Endothelial Cells From Umbilical Cord Veins
- FGF
Fibroblast growth factor
- EGF
Epidermal Growth Factor
- FCS
Fetal Calf Serum
- NCS
Newborn Calf Serum
- HBS
HEPES-Buffered Saline
- ECM
Extracellular Matrix
- LHM
Peptide PyroGlu-His-Ser-Phe-Thr-Ile-Lys-Ile-ThrCONH2
- IF
1:1 mixture of Iscove's MDM and F12 basal media |
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Keywords: | endothelium tissue culture technique clonal growth fibroblast growth factor transferrin heparin |
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