Rapid immunodetection of Escherichia coli |
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| Authors: | Alice R Arredondo Brent L Dorval Alexander M Klibanov Kim Lewis |
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| Institution: | (1) Biotechnology Center, Tufts University, Medford, MA 02155, USA;(2) Oemconcept, 1 Business Way, Hopedale, MA 01747, USA;(3) Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA |
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| Abstract: | A filtration flow-through design was used to develop the rapid immunodetection of Escherichia coli. Polyclonal anti-E. coli IgG was conjugated to small, 0.8  Blue latex beads. Cells were mixed with conjugated beads in the presence of anti-E. coli monoclonal IgM. The suspension was then filtered through a 5 nitrocellulose membrane. The cell-containing complexes were effectively collected on the filter, forming a blue spot. The method produced reliable detection of E. coli at a concentration of 105 cells ml–1, which is a current benchmark figure for urinary tract infection (UTI) diagnosis. |
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| Keywords: | bacteria immunodetection urinary tract infection |
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