Pulvinus: an ideal explant for plant regeneration in <Emphasis Type="Italic">Caesalpinia bonduc</Emphasis> (L.) Roxb., an important ethnomedicinal woody climber |
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Authors: | Meena K Cheruvathur John Britto T Dennis Thomas |
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Institution: | (1) Postgraduate and Research Department of Botany, St. Thomas College, Pala, Arunapuram (P.O), Kottayam, 686 574, Kerala, India;(2) The Rapinat Herbarium and Centre for Molecular Systematics and Taxonomy, St. Joseph’s College (Autonomous), Tiruchirappalli, 620 002, Tamil Nadu, India; |
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Abstract: | This study demonstrates the morphogenic potential of pulvinus, an important organ situated at the base of the petiole or rachis
of leguminous plants. Plant regeneration via pulvinus-derived calli of Caesalpinia bonduc has been achieved. Organogenic calli have been derived from the explant 45 days after culture on Murashige and Skoog (MS)
medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with 6-benzylaminopurine (BA). Optimum
callus induction (100%) occurred when the pulvini were cultured on MS medium fortified with 6 mg l−1 2,4-D and 1 mg l−1 BA. The highest shoot induction was obtained when the calli were transferred to MS medium supplemented with 5 mg l−1 BA and 1 mg l−1 indole-3-acetic acid (IAA). On this medium, 87% cultures responded with an average number of 4.2 shoots per culture. The
maximum root induction from the regenerated shoots was observed on half strength MS medium containing 6 mg l−1 indole-3-butyric acid (IBA). Here 100% shoots rooted with a mean number of 6.3 roots per shoot. The regenerated plantlets
were acclimatized and subsequently showed normal growth. This efficient protocol will be helpful for propagating elite clones
on a mass scale and could be utilized for genetic transformation study. |
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