Bleomycin resistance: a new dominant selectable marker for plant cell transformation |
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| Authors: | Jacques Hille Frank Verheggen Peter Roelvink Henk Franssen Ab van Kammen Pim Zabel |
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| Affiliation: | (1) Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands |
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| Abstract: | Summary Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens. The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells. |
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| Keywords: | bleomycin selectable marker A. tumefaciens plant transformation |
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