Bleomycin resistance: a new dominant selectable marker for plant cell transformation |
| |
| Authors: | Jacques Hille Frank Verheggen Peter Roelvink Henk Franssen Ab van Kammen Pim Zabel |
| |
| Institution: | (1) Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands |
| |
| Abstract: | Summary Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens. The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells. |
| |
| Keywords: | bleomycin selectable marker A tumefaciens plant transformation |
| 本文献已被 SpringerLink 等数据库收录！ |
