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Protoplasts from the cyanobacterium,Spirulina platensis
Authors:A M Abo-Shady  S M Abou-El-Souod  Abd El-Raheem  R El-Shanshoury  Y A G Mahmoud
Institution:(1) Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
Abstract:Protoplasts were obtained from the filamentous blue-green algaSpirulina platensis by treating the filaments with 0.05% (w/v) lysozyme in 0.03m phosphate buffer. The protoplasts regenerated cell walls and formed colonies when plated on a regeneration medium. The highest percentage of regeneration, 40% was obtained after 21 days.
Keywords:Lysozyme  protoplasts  regeneration  Spirulina platensis
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