Compartmentation of newly synthesized phosphatidylethanolamine in rat brain microsomes |
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Authors: | Luciano Binaglia Rita Roberti Louis Freysz Giuseppe Arienti Lanfranco Corazzi Giuseppe Porcellati |
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Institution: | (1) Department of Biological Chemistry, The Medical School, University of Perugia, 06100 Perugia, Italy;(2) Centre de Neurochimie du CNRS, U44 de l'INSERM, 67084 Strasbourg Cedex, France |
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Abstract: | Summary The compartmentation of the phosphatidylethanolamine newly synthesized in brain microsomesin vitro either by base exchange or net synthesis has been studied, using difluorodinitrobenzene as a chemical probe. The experimental results demonstrate that in rat brain microsomes the phosphatidylethanolamine molecules synthesized by base exchange and the bulk membrane lipid belong to different pools. Ca2+ bound to microsomes seems to be involved in the maintenance of the compartmentation of phosphatidylethanolamine. In the presence of Ca2+ the newly synthesized phosphatidylethanolamine molecules react with difluorodinitrobenzene as though they are organized in clusters. After biosynthesisin vivo orin vitro through the cytidine pathway, the compartmentation of the newly formed phosphatidylethanolamine appears less marked than after the synthesis through base exchange. |
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Keywords: | phosphatidylethanolamine cross-linking brain microsomes divalent cations |
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