Validation of a simplified method for determination of cimetidine in human plasma and urine by liquid chromatography with ultraviolet detection |
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| Authors: | Iqbal Tahira Karyekar Chetan S Kinjo Minori Ngan Gilbert C Dowling Thomas C |
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| Institution: | Renal Clinical Pharmacology Lab, Department of Pharmacy Practice and Science, University of Maryland, School of Pharmacy, 100 N. Penn St., AHB Rm. 540-D, Baltimore, MD 21201, USA. |
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| Abstract: | A HPLC method was developed for determination of cimetidine in human plasma and urine. Plasma samples were alkalinized followed by liquid extraction with water-saturated ethyl acetate then evaporated under nitrogen. The extracts were reconstituted in mobile phase and injected onto a C(18) reversed-phase column; UV detection was set at 228 nm. Urine samples were diluted with an internal standard/mobile phase mixture (1:9) prior to injection. The lower limit of quantification in plasma and urine were 100 ng/ml and 10 microg/ml, respectively; intra- and inter-day coefficients of variation were
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