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Related mannose-specific lectins from different species of the family Amaryllidaceae
Authors:Els J M Van Damme  Anthony K Allen  Willy J Peumans
Abstract:Bulbs from three species of the plant family Amaryllidaceae ( Narcissus pseudonurcissus L., Leucojum aestivum L. and Leucojum vernum L.) were found to contain mannose-specific lectins. These lectins were serologically identical to a previously reported Amaryllidaceae lectin from Galanthus nivalis L. bulbs, but had a different molecular structure. The lectins described in this paper are dimeric proteins composed of subunits of 13 kDa, which are not held together by disulphide bridges. In hapten-inhibition assays Amaryllidaceae lectins exhibited exclusive specificity towards mannose. Furthermore, they all had a high specific agglutination activity with trypsin-treated rabbit erythrocytes, whereas human red blood cells were not agglutinated.
Keywords:Amaryllidaceae  Galanthus nivalis  lectin  Leucojum aestivum  Leucojum vernum  mannose  Narcissus pseudonarcissus
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