Isolation and characterization of 12 polymorphic microsatellite markers from ladyfish (Elops saurus Linnaeus) |
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Authors: | Zhen-Xia Sha Shi-Chao Xing Chang-Wei Shao Yong-Sheng Tian Xiao-Lin Liao Song-Lin Chen |
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Affiliation: | (1) Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, 266071 Qingdao, China;(2) College of Marine Life Sciences, Ocean University of China, 266003 Qingdao, China; |
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Abstract: | Ladyfish (Elops saurus Linnaeus) is an economically important marine fish species. 76 microsatellite loci were isolated from an enriched genomic library of Elops saurus. Twelve of these markers were polymorphic in a test population with alleles per locus ranging from three to nine. The number of observed, expected heterozygosity and polymorphism information content (PIC) per locus in 20 individuals ranged from 0.2000 to 1.0000, 0.1897–0.8846, 0.1769–0.8476, respectively. One markers significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found between pairs of markers. As a result, 12 microsatellite markers probably should provide sufficient level of genetic diversity to investigate the fine-scale population structure, stock management and enhancement, genetic linkage map construction and molecular marker-assisted breeding in Elops saurus Linnaeus. |
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