Further purification of a fibroblast growth factor-like factor from chick embryo extract by heparin-affinity chromatography |
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Authors: | Ichiro Kimura Yae Gotoh Eijiro Ozawa |
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Institution: | (1) Division of Cell Biology, National Institute of Neuroscience, National Center for Neurology and Psychiatry, Kodaira, 187 Tokyo, Japan;(2) Present address: Department of Basic Human Sciences, School of Human Sciences, Waseda University, Tokorozawa, 359 Saitama, Japan |
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Abstract: | Summary A mitogenic factor which promotes quail myoblast proliferation has been purified some 105-fold from chick embryo extract by a combination of cation-exchange chromatography and heparin-affinity chromatography. The
factor is eluted from heparin-Sepharose with 2M NaCl and is a single-chain polypeptide with an apparent molecular weight of 15000 to 17000. It is active at subnanogram level
in triggering the proliferation and thereby delaying temporarily fusion of myoblasts. It also stimulates the proliferation
of quail fibroblasts in a similar effective concentration range. For both myoblasts and fibroblasts the dose-response to the
factor is quantitatively and qualitatively comparable with that of bovine pituitary fibroblast growth factor. These observations
strongly suggest that the factor very probably corresponds to chicken fibroblast growth factor or to a closely related molecule(s)
and that it is possibly involved in the regulation of myogenesis.
This work was partly supported by a grant from the National Center of Neurology and Psychiatry (NCNP grant 86-01) of the Ministry
of Health and Welfare, Japan. |
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Keywords: | FGF growth factor myoblast proliferation myogenesis embryo extract |
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