Isolation and characterization of an apoprotein from the d less than 1.006 lipoproteins of human and canine lymph homologous with the rat A-IV apoprotein. |
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| Authors: | K H Weisgraber T P Bersot R W Mahley |
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| Affiliation: | 1. Instituto de Química, Department of Biochemistry, Universidade de São Paulo, C.P. 20780, São Paulo, Brazil;2. Instituto de Química, Universidade Estadual de Campinas, C.P. 1170, Campinas, São Paulo, Brasil. |
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| Abstract: | The singlet oxygen traps, 2,5-diphenylfurane and 1,3-diphenylisobenzofurane were oxidized to cis-benzoylethylene and o-dibenzoylbenzene during the decomposition of diisopropyl-N-nitrosamine catalyzed by peroxidase. Singlet oxygen quenchers inhibited this conversion and also the chemiluminescence accompaying the catalyzed reaction. The chemiluminescence is enhanced by 1,4-diazobicyclo (2.2.2) octane, fluorescein, eosin rhodamine B and rose bengal but little effect was detected in the presence of 9,10-dibromoanthracene-2-sulfonate, 9,10-diphenylanthracene-2-sulfonate and anthracene-2-sulfonate. An emission spectrum of the unsensitized reaction in 560 – 600 nm region was observed. It is concluded that singlet oxygen is formed during peroxidase catalyzed degradation of diisopropyl-N-nitrosamine. |
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| Keywords: | To whom inquires should be addressed. |
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