The uptake of copper by cell suspension cultures of Phaseolus vulgaris L. cv. SAXA

In experiments with cell suspension cultures of Phaseolus vulgaris L. cv. SAXA it could be demonstrated that gas-shocks caused by aeration decrease the uptake of copper. An aeration period of 30 h prior to the addition of copper was found to be sufficient to reestablish the copper uptake system. EDTA/sucrose solutions seemed to be adequate to remove the adsorbed copper. The time course of the uptake of copper showed saturation behaviour, whereas the velocity of the uptake was dependent on the given concentration of copper.