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| 龙眼花芽ANS基因的克隆与原核表达 | |
| 引用本文： | 游向荣,许鸿川,梁文裕,陈清西,郑少泉,陈伟.龙眼花芽ANS基因的克隆与原核表达[J].热带亚热带植物学报,2010,18(3):288-292. |
| 作者姓名： | 游向荣 许鸿川 梁文裕 陈清西 郑少泉 陈伟 |
| 作者单位： | 1. 福建农林大学,作物遗传育种与综合利用教育部重点实验室,福州,350002;福建农林大学,生命科学学院,福州,350002 2. 福建农林大学,生命科学学院,福州,350002 3. 福建农林大学,园艺学院,福州,350002 4. 福建省农业科学院果树研究所,福州,350013 |
| 基金项目： | 国家自然科学基金，教育部高等学校博士学科点专项科研基金，福建自然科学基金 |
| 摘 要： | 运用蛋白质组学比较龙眼(Dimocarpus hngan Lour.)正常成花和成花逆转花芽的蛋白质组变化,结果表明,ANS蛋白(anthocyanidin synthase)在龙眼成花逆转花芽中下调表达.应用RACE方法克隆ANS蛋白的全长cDNA,长度为1477 bp,包括1个1071 bp的开放阅读框,编码357 bp的氨基酸序列,GenBmk的登录号为FJ479616(GI:218202927).将ANS在大肠杆菌中表达,获得1个约46 kD的外源蛋白.这说明ANS蛋白在龙眼成花逆转过程中起作用. |
| 关 键 词： | 龙眼 成花逆转 差异蛋白 克隆 表达 |
| 收稿时间： | 2009/5/11 0:00:00 |
| 修稿时间： | 2009/11/15 0:00:00 |
Cloning and Prokaryotic Expression of Longan ANS Gene |
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| YOU Xiang-rong,XU Hong-chuan,LIANG Wen-yu,CHEN Qing-xi,ZHENG Shao-quan,CHEN Weia.Cloning and Prokaryotic Expression of Longan ANS Gene[J].Journal of Tropical and Subtropical Botany,2010,18(3):288-292. | |
| Authors: | YOU Xiang-rong XU Hong-chuan LIANG Wen-yu CHEN Qing-xi ZHENG Shao-quan CHEN Weia |
| Institution: | Key Laboratory of Crop Genetics and Multipurpose Utilization, Ministry of Education, Fujian Agriculture and Forestry University; College of Life Science, Fujian Agriculture and Forestry University,College of Life Science, Fujian Agriculture and Forestry University,College of Life Science, Fujian Agriculture and Forestry University,College of Horticulture, Fujian Agriculture and Forestry University,Fruit Research Institute, Fujian Academy of Agriculture Science,Key Laboratory of Crop Genetics and Multipurpose Utilization, Ministry of Education, Fujian Agriculture and Forestry University; College of Life Science, Fujian Agriculture and Forestry University |
| Abstract: | The changes in proteome of normal flower and floral reversion buds in longan (Dimocarpus longan Lour.) were studied by using Proteomics method. The results showed that ANS (anthocyanidin synthase) protein was found down-regulated expression in floral reversion buds. ANS cDNA was obtained (GenBank access number: FJ479616) by using RACE method, its full length was 1477 bp, with a 1071 bp open read frame. ANS gene was expressed in E. coli, and a 46 kD heterologous protein was obtained. It suggested that ANS protein play a role in floral reversion in longan. |
| Keywords: | ANS |
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