The economics of inclusion body processing |
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| Authors: | G H Lee D Cooney A P J Middelberg W S Choe |
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| Institution: | (1) Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, 117576 Singapore, Singapore;(2) Department of Chemical Engineering, University of Cambridge, Pembroke Street, CB2 3RA Cambridge, UK;(3) Centre for Biomolecular Engineering, Division of Chemical Engineering, University of Queensland, 4072 St Lucia, QLD, Australia;(4) Department of Chemical Engineering, Sungkyunkwan University, 440-746 Suwon, Korea |
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| Abstract: | Many recombinant proteins are often over-expressed in host cells, such as Escherichia coli, and are found as insoluble and inactive protein aggregates known as inclusion bodies (IBs). Recently, a novel process for IB extraction and solubilisation, based on chemical extraction, has been reported. While this method has the potential to radically intensify traditional IB processing, the process economics of the new technique have yet to be reported. This study focuses on the evaluation of process economics for several IB processing schemes based on chemical extraction and/or traditional techniques. Simulations and economic analysis were conducted at various processing conditions using granulocyte macrophage-colony stimulating factor, expressed as IBs in E. coli, as a model protein. In most cases, IB processing schemes based on chemical extraction having a shorter downstream cascade demonstrated a competitive economic edge over the conventional route, validating the new process as an economically more viable alternative for IB processing. |
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