CXCR3/CXCL10 expression in the synovium of children with juvenile idiopathic arthritis |
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Authors: | Email author" target="_blank">Georgia?MartiniEmail author Francesco?Zulian Fiorella?Calabrese Marta?Bortoli Monica?Facco Anna?Cabrelle Marialuisa?Valente Franco?Zacchello Carlo?Agostini |
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Institution: | (1) Department of Paediatrics, Padua University School of Medicine, Italy;(2) Pathology Institute, Padua University School of Medicine, Italy;(3) Department of Clinical and Experimental Medicine, Padua University School of Medicine, Italy |
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Abstract: | The accumulation of T cells in the synovial membrane is the crucial step in the pathophysiology of the inflammatory processes
characterizing juvenile idiopathic arthritis (JIA). In this study, we evaluated the expression and the pathogenetic role in
oligoarticular JIA of a CXC chemokine involved in the directional migration of activated T cells, i.e. IFNγ-inducible protein
10 (CXCL10) and its receptor, CXCR3. Immunochemistry with an antihuman CXCL10 showed that synovial macrophages, epithelial
cells, and endothelial cells bear the chemokine. By flow cytometry and immunochemistry, it has been shown that CXCR3 is expressed
at high density by virtually all T lymphocytes isolated from synovial fluid (SF) and infiltrating the synovial membrane. Particularly
strongly stained CXCR3+ T cells can be observed close to the luminal space and in the perivascular area. Furthermore, densitometric analysis has
revealed that the mRNA levels for CXCR3 are significantly higher in JIA patients than in controls. T cells purified from SF
exhibit a definite migratory capability in response to CXCL10. Furthermore, SF exerts significant chemotactic activity on
the CXCR3+ T-cell line, and this activity is inhibited by the addition of an anti-CXCL10 neutralizing antibody. Taken together, these
data suggest that CXCR3/CXCL10 interactions are involved in the pathophysiology of JIA-associated inflammatory processes,
regulating both the activation of T cells and their recruitment into the inflamed synovium. |
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