Oxygen availability for bacterial growth with a flow microcalorimeter |
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| Authors: | J P Herman E Jakubczak J P Belaich H Leclerc |
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| Institution: | (1) I.N.S.E.R.M. (U. 146), Domaine du C.E.R.T.I.A., 59651 Villeneuve d'Ascq, Cedex, France;(2) E.N.S.I.A., Domaine du C.E.R.T.I.A., 59651 Villeneuve d'Ascq, Cedex, France;(3) Institut Pasteur de Lille, Lille, France;(4) Chimie bactérienne, C.N.R.S., Marseille;(5) U.E.R. des Sciences Médicales, Université de Lille II, Lille, France |
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| Abstract: | Summary The enthalpy change associated with aerobic growth of E. coli K12 on minimal media with succinic acid as sole carbon and energy source, determined by flow microcalorimetry (with aerobic mixing cell) was 733.01±15.32 kJ·mol–1. Molar growth yield was 39.6±1.2 g·mol–1. When the microcalorimetric growth was limited by oxygen supply, the power-time curve was altered and the total heat evolved was less than the enthalpy change. The maximum thermal output corresponding to a fully aerobic growth in the calorimetric cell was 1.89×10–3 W·ml–1. Thus, the oxygen uptake rate was about 0.39 ml O2·h–1·ml–1. |
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