Screening and mutagenesis of moulds for the improvement of glucose oxidase production |
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Abstract: | The strain of Aspergillus niger G most effective for producing glucose oxidase (see β-
-glucose:oxygen 1-oxidoreductase, EC 1.1.3.4) was selected out of 110 moulds belonging to 15 different species by the method of test-tube microculture. Conidia of the selected strain were further subjected to mutagenesis with both u.v. and N-methyl-N′-nitro-N-nitrosoguanidine (NTG) and the products were analysed for glucose oxidase activity with our own diffusion plate method. Among 960 strains isolated after mutagenesis only 12 showed higher activity (from 1.5 to 18%) than the starting strain A. niger G. |
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Keywords: | Aspergillus niger G glucose oxidase screening mutagenesis |
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