Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA |
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| Authors: | Crispin J. Woolston Richard Barker Helen Gunn Margaret I. Boulton Philip M. Mullineaux |
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| Affiliation: | (1) AFRC Institute of Plant Science Research, John Innes Institute, Colney Lane, NR4 7UH Norwich, Norfolk, UK;(2) Sequencing Systems Limited, Unit 184, Cambridge Science Park, CB4 4GN Cambridge, UK |
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| Abstract: | ![]()
Cloned DNA of the geminivirus wheat dwarf virus (WDV) was successfully used to infect seedling wheat plants. The clone was derived from circular double-stranded viral DNA isolated from naturally infected tissue. The initiation of infection was mediated by Agrobacterium tumefaciens using cloned dimeric WDV genomes in a binary Agrobacterium vector. The WDV DNA which comprised the infectious clone was sequenced and is compared with the published sequence of a Swedish isolate of the same virus. The results confirm that the single WDV genome component of 2.75 kb carries all the information necessary for production of viral symptoms, virus particles and viral double- and single-stranded DNA forms. |
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| Keywords: | Agrobacterium tumefaciens Agroinfection Geminivirus Wheat Dwarf Virus |
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