Determination of gentiopicroside contents during somatic embryogenesis in Gentiana straminea Maxim |
| |
Authors: | Lina Yang Haitao Wang Tao He |
| |
Institution: | 1. School of Ecol-Environmental Engineering, Qinghai University, Xining, 810016, China 2. State Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm, Qinghai University, Xining, 810016, China
|
| |
Abstract: | A protocol for regeneration of Gentiana straminea Maxim recently established in our laboratory, somatic embryogenesis was obtained from its leaf derived calli. The gentiopicroside contents of embryogenic calli, globular-, heart-, torpedo-, and cotyledon-shaped embryoids as well as regenerated plantlets were determined by high-performance liquid chromatography. Gentiopicroside was detectable in all materials tested. Embryogenic calli showed the lowest gentiopicroside content. The changes of gentiopicroside contents were not significant (P < 0.05) with the development of somatic embryos. The highest gentiopicroside content (30.7 mg g?1 dry weight) was achieved in regenerated plantlets. The contents of gentiopicroside were not significant (P < 0.05) differences between control plants and embryogenic calli, different stages of somatic embryos and regenerated plantlets. This protocol could be employed for producing gentiopicroside or other medicinal compounds. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|