| 青霉素过敏人群IL-18血清浓度以及启动子区基因多态性研究 |
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| 引用本文： | 畅灵丽,;窦会娟,;陈旭东,;赵明一.青霉素过敏人群IL-18血清浓度以及启动子区基因多态性研究[J].激光生物学报,2014(4):374-379. |
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| 作者姓名： | 畅灵丽 ;窦会娟 ;陈旭东 ;赵明一 |
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| 作者单位： | [1]漯河医学高等专科学校微生物与免疫学教研室,河南漯河462000; [2]漯河医学高等专科学校组织胚胎学教研室,河南漯河462000; [3]中南大学湘雅三医院儿科,湖南长沙410013 |
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| 基金项目： | 河南省科技厅自然科学基金研究项目（132300410170） |
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| 摘 要： | 目的：探讨青霉素类抗生素过敏反应与IL-18及其基因多态性的关系。方法：采用ELISA方法检测50例正常对照和67例过敏患者血清中IL-18水平,PCR-SSP法检测IL-18启动子区多态性位点的基因型。结果：青霉素过敏组IL-18血清浓度显著高于正常对照组（P〈0.01）,且随着皮试反应程度的增强,IL-18浓度也随之升高;青霉素过敏病人IL-18-607位点A等位基因出现频率显著高于对照组（P〈0.01）,CA＋CC基因型降低了青霉素过敏发生的风险性2=5.868,P〈0.05,OR=3.910,95%Cl（1.225,12.478）]。不同基因型患者血清中IL-18浓度无显著性差异。结论：青霉素过敏反应与IL-18升高有关,IL-18基因启动子区-607C/A位点多态性与青霉素过敏显著相关。
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| 关 键 词： | 青霉素过敏 IL-18 基因多态性 |
The Expression of IL-18 and Its Promoter Polymorphism in Patients with Allergic Reaction to Penicillin |
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| Institution: | CHANG Lingli , DOU Huijuan , CHEN Xudong, ZHAO Mingyi ( 1. Department of Microbiology and Immunology, Luohe Medical College, Luohe 462000, Henan, Chi 2. Department of Embryology, Luohe Medical College, Luohe 462000, Henan, China; 3. Department of Pediatrics, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan, |
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| Abstract: | Objective： To analyze the relationship between interleukin-8（ IL-8） in serum and penicillin allergy and the correlation between allergic reaction to penicillin and IL-8 candidate gene polymorphism. Method： The serum levels of IL-18 in 50 control subjects and 67 patients with allergic reaction to penicillin were measured by ELISA. Single nucleotide polymorphisms（ SNPs） of IL-18 genes were detected by polymerase chain reaction with sequence-specific primer（ PCR-SSP）. Results： There were significantly higher IL-18 levels in allergic patients compared to normal controls（ P〈 0. 01）. IL-18 level appeared a significant difference between positive and control groups（ P〈 0. 01）. At IL-18-607 A / C position,the A allele occurred more frequently in patients group than that in the controls（ P〈 0. 01）. CA ＋ CC genotype decreased the risk of allergic reaction to penicillin. 2 = 5. 868,P〈 0. 05,OR = 3. 910,95% Cl（ 1. 225,12. 478） ]. Specific IL-18 level did not give significant difference between patients with different genotype.Conclusion： The result showed that the increase of IL-18 level might be associated with allergic reaction to penicillin.IL-18 promoter-607 C / A polymorphism is significantly associated with the occurrence of penicillin allergy. |
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| Keywords: | Penicillin allergy IL-18 gene polymorphisms |
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