| 可诱导表达Mesp1的慢病毒载体构建及P19细胞稳定表达系的建立 |
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| 引用本文： | 宋文,高建芳,陈辉,邓云,李永青,袁婺洲,吴秀山,戴国,王跃群. 可诱导表达Mesp1的慢病毒载体构建及P19细胞稳定表达系的建立[J]. 激光生物学报, 2014, 0(4): 351-355 |
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| 作者姓名： | 宋文 高建芳 陈辉 邓云 李永青 袁婺洲 吴秀山 戴国 王跃群 |
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| 作者单位： | 湖南师范大学蛋白质化学及鱼类发育生物学教育部重点实验室,心脏发育研究中心,湖南长沙410081 |
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| 基金项目： | 湖南省科技计划项目资助（编号：2013RS4034）; 国家自然科学基金面上项目（编号：31272396）; 湖南省生物发育工程及新产品研发协同创新中心（2013-448-6） |
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| 摘 要： | Mesp1属于b HLH转录因子家族,对早期心脏中胚层的形成十分重要。为研究Mesp1在心脏发育过程中的功能,本文构建了可诱导表达Mesp1的慢病毒载体,转染进P19细胞,经嘌呤霉素筛选并扩大培养后,成功建立起经强力霉素诱导后可稳定过表达Mesp1基因的细胞系P19-Mesp1,为研究Mesp1心脏发育相关功能提供有用的细胞研究模型。
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| 关 键 词： | 可诱导慢病毒载体 Mesp1 强力霉素 P19 稳定细胞系 |
Construction of Inducible Lentiviral Vector Containing Mesp1 and Establishment of a Stably Expressing Mesp1 P19 Cell Line |
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| SONG Wen,GAO Jianfang,CHEN Hui,DENG Yun,LI Yongqing,YUAN Wuzhou,WU Xiushan,DAI Guo ＊,WANG Yuequn. Construction of Inducible Lentiviral Vector Containing Mesp1 and Establishment of a Stably Expressing Mesp1 P19 Cell Line[J]. ACTA Laser Biology Sinica, 2014, 0(4): 351-355 |
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| Authors: | SONG Wen GAO Jianfang CHEN Hui DENG Yun LI Yongqing YUAN Wuzhou WU Xiushan DAI Guo ＊ WANG Yuequn |
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| Affiliation: | ＊ (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, Hunan Normal University, Changsha410081, Hunan, China) |
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| Abstract: | The Mesp1 gene encodes the basic HLH protein Mesp1 which is expressed in the mesodermal cell lineage during early gastrulation. In order to study the function of Mesp1 in heart development,inducible lentiviral vector containing Mesp1 was constructed,then it was tranfected into P19 cells to construct a stably Mesp1-expressing P19 cell lines.After screening culture by puromycin,the over expression of Mesp1 was verified by RT-PCR and Western blotting. The P19 cells line that over-express Mesp1 stably was established successfully,which provide a cell model for studying the molecular function of Mesp1 in heart development. |
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| Keywords: | inducible lentiviral vector Mesp1 DOX P19 stable cell line |
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