Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains |
| |
Authors: | Stephen P Slocombe QianYi Zhang Kenneth D Black John G Day Michele S Stanley |
| |
Institution: | 1. Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll, PA37 1QA, UK
|
| |
Abstract: | The phenotypic and phylogenetic diversity of micro-algae capable of accumulating triacylglycerols provides a challenge for the accurate determination of biotechnological potential. High-yielding strains are needed to improve economic viability and their compositional information is required for optimizing biodiesel properties. To facilitate a high-throughput screening programme, a very rapid direct-derivatization procedure capable of extracting lyophilized material for GC analysis was compared with a scaled-down Folch-based method. This was carried out on ten micro-algal strains from 6 phyla where the more rapid direct-derivatization approach was found to provide a more reliable measure of yield. The modified Folch-based procedure was found to substantially underestimate oil yield in one Chlorella species (P?<?0.01). In terms of fatty acid composition however, the Folch procedure proved to be slightly better in recovering polyunsaturated fatty acids, in six out of the ten strains. Therefore, direct-derivatization is recommended for rapid determination of yields in screening approaches but can provide slightly less compositional accuracy than solvent-based extraction methods. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|