Cytochromebc purified from the methanogenMethanosarcina barkeri |
| |
| Authors: | Kumazawa Youichi Fujiwara Taketomo Fukumori Yoshihiro Koga Yosuke Yamanaka Tateo |
| |
| Institution: | (1) Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan;(2) Department of Chemistry, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan;(3) Department of Industrial Chemistry, College of Science and Technology, Nihon University, Kanda-Surugadai 1-5, Chlyoda-ku, 101 Tokyo, Japan |
| |
| Abstract: | Cytochromebc was partially purified from the methanogen,Methanosarcina barkeri. The cytochrome was composed of three subunits with molecular masses of 23.4, 20.9, and 9.1 kDa, respectively, and the 23.4 kDa subunit contained haemc. The absorption spectrum of cytochromebc showed a peak at 411 nm in the oxidized form, and peaks at 554, 524, and 422 nm in the reduced form. The cytochrome reacted with CO, and its low temperature absorption spectrum showed the  peak at 552 nm with a shoulder at 557 nm. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
