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Activation of fatty acids by non-glyoxysomal peroxisomes
Authors:H. Gerbling  B. Gerhardt
Affiliation:(1) Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland
Abstract:Ethylene treatment (approx. 20 mgrl ·1-1 in air for 2 d) of tobacco (Nicotiana tabacum L. cv. Havana 425) plants markedly increases the endo-beta-1,3-glucanase (EC 3.2.1.39) content of leaves. The antigenic form of the enzyme induced is the same one whose production is blocked by treating cultured cells with combinations of auxin (1.1 · 10-5 M agr-naphthaleneacetic acid) and cytokinin (1.4 · 10-6 M kinetin). Evidence is presented that cultured tobacco cells require ethylene for beta-1,3-glucanase accumulation: i) ethylene treatment increased the accumulation of -1,3-glucanase in callus tissues >10 d after subculturing and in cell-suspension cultures; ii) callus tissues can produce ethylene; iii) conditions known to inhibit ethylene production (1 mM CoCl2; 33° C treatment) or ethylene action (approx. 1.6 mmol · 1-1 norbornadiene in air) inhibited beta-1,3-glucanase accumulation by callus tissues treated for 4 d following subculturing; and, these inhibitory effects were prevented by exogenous ethylene. Combinations of auxin and cytokinin blocked ethylene-induced accumulation of beta-1,3-glucanase by cell-suspension cultures. The results favor a model in which ethylene induces results favor a model in which ethylene induces beta 1,3-glucanase accumulation, and auxin and cytokinin inhibit this induction process.Abbreviations NAA agr-naphthaleneacetic acid - NDE norbornadiene
Keywords:Auxin and ethylene action  Cell culture (ethylene,   /content/jq1705n664310m00/xxlarge946.gif"   alt="  beta"   align="  MIDDLE"   BORDER="  0"  > 1,3-glucanase accumulation)  Cytokinin and ethylene action  Ethylene (glucanohydrolase-regulated)    /content/jq1705n664310m00/xxlarge946.gif"   alt="  beta"   align="  MIDDLE"   BORDER="  0"  >-1,3-Glucanase-Nicotiana
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