A Method for Isolation of Pasteuria penetrans Endospores for Bioassay and Genomic Studies |
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| Authors: | Jenora T Waterman David McK Bird and Charles H Opperman |
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| Institution: | Center for the Biology of Nematode Parasitism, NC State University, Raleigh, NC 27695 |
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| Abstract: | A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations. |
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| Keywords: | endospores method Pasteuria penetrans |
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