2-Ketogluconic Acid Production and Phosphate Solubilization by Enterobacter intermedium |
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| Authors: | Hoon Hwangbo Ro Dong Park Yong Woong Kim Yo Sup Rim Keun Hyung Park Tae Hwan Kim Jang Sun Suh Kil Yong Kim |
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| Affiliation: | (1) Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture, Chonnam National University, Gwangju 500-757, Korea, KR;(2) Department of Agricultural Chemistry, Sunchon National University, Sunchon 540-742, Korea, KR;(3) Department of Animal Science, Chonnam National University, Gwangju 500-757, Korea, KR;(4) National Institute of Agricultural Science and Technology, Suwon 440-707, Korea, KR |
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| Abstract: | Enterobacter intermedium, isolated from grass rhizosphere, exhibited a strong ability to solubilize insoluble phosphate. This bacterium oxidized glucose to gluconic acid and sequentially to 2-ketogluconic acid (2-KGA), which was identified using HPLC and GC-MS. The ability of E. intermedium to solubilize phosphate and produce 2-KGA produce in broth medium containing different components was monitored with air and without air supply. With an air supply, the production of 2-KGA markedly increased to about 110 g/l at day 10 in media containing 0.2 M gluconic acid, while it was about 65 g/l without gluconic acid addition. With an air supply, the concentration of soluble phosphate significantly decreased to 200–250 mg/l in media containing 1% CaCO3, whereas it was about 1000 mg/l without CaCO3 addition. Without an air supply, the concentration of 2-KGA and phosphate were negligible throughout the culture period. RID= ID= Correspondence to: K.Y. Kim; email: kimkil@chonnam.ac.kr Received: 21 August 2002 / Accepted: 25 September 2002 |
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