Effect of doxorubicin on the intestinal membranes in rats—Influence of α-tocopherol administration |
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Authors: | A Geetha R Sankar Thankamani Marar C S Shyamala Devi |
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Institution: | (1) Department of Biochemistry, University of Madras, Guindy Campus, 600 025 Madras, India |
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Abstract: | The effect of α-tocopherol on doxorubicin induced changes in intestinal brush border and basolateral membranes were studied
in rats. Rats were treated with doxorubicin (2.5 mg/kg body wt.), intravenously, weekly for 8 weeks. α-Tocopherol (400 mg/kg
body wt.) was given orally, daily for 2 months. Intestinal basolateral membrane bound ATPases and brush border membrane bound
alkaline phosphatase activities were found to be decreased significantly in doxorubicin treated rats. The lipid peroxide level
was found to be elevated with a significant depletion in membrane sulphydryl groups. In α-tocopherol coadministered animals,
the enzyme activities were found to be restored with concomitant reduction in lipid peroxide levels and an increase in the
membrane sulphydryl groups. The membrane cholesterol and phospholipid levels which were altered in doxorubicin treated animals
were found to be maintained significantly. The results are discussed with reference to the effect of α-tocopherol on lipid
peroxidation and membrane sulphydryl groups. |
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Keywords: | Doxorubicin intestine basolateral membrane brush border membrane ATPases alkaline phosphatase sulphydryl groups α -tocopherol antioxidant |
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