Synchronization of drosophila cells in culture |
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| Authors: | Angie Rizzino Alan B Blumenthal |
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| Institution: | (1) Laboratory of Radiobiology, University of California, 94143 San Francisco, California;(2) Present address: Biology Department, Muir College, University of California, San Diego, 92038 La Jolla, California |
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| Abstract: | Summary We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells
of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population
accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.
This work was performed under the auspices of the U.S. Energy Research and Development Administration. A.R. was supported
by an NIH post-doctoral fellowship, No. CA01060. |
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| Keywords: | Drosophila cell lines cell synchrony cell cycle S phase |
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