Ring dechlorination of deethylsimazine by hydrolases from Rhodococcus corallinus |
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Authors: | Alasdair M Cook R Hütter |
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Institution: | Department of Microbiology, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zürich, Switzerland |
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Abstract: | Abstract Cell-free extracts of Rhodococcus corallinus NRRL B-15444R dechlorinated deethylsimazine (2-chloro-4-ethylamino-1,3,5-triazine-6-amine) to N -ethylammeline (2-amino-4-ethylamino-1,3,5-triazine-6(5H)-one) anaerobically, and, thus, presumably hydrolytically. Two enzyme fractions ( M r about 450 000 and 180 000) that dechlorinated deethylsimazine were detected after gel permeation chromatography of cell extracts. |
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Keywords: | s-Triazines degradative pathway isozymes |
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