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Ring dechlorination of deethylsimazine by hydrolases from Rhodococcus corallinus
Authors:Alasdair M Cook  R Hütter
Institution:Department of Microbiology, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zürich, Switzerland
Abstract:Abstract Cell-free extracts of Rhodococcus corallinus NRRL B-15444R dechlorinated deethylsimazine (2-chloro-4-ethylamino-1,3,5-triazine-6-amine) to N -ethylammeline (2-amino-4-ethylamino-1,3,5-triazine-6(5H)-one) anaerobically, and, thus, presumably hydrolytically. Two enzyme fractions ( M r about 450 000 and 180 000) that dechlorinated deethylsimazine were detected after gel permeation chromatography of cell extracts.
Keywords:s-Triazines  degradative pathway  isozymes
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