Evidence for phospholipid in plasma membrane penicillinase of |
| |
Authors: | Tetsuo Sawai Laura J Crane JO Lampen |
| |
Institution: | Institute of Microbiology, Rutgers University, The State University of New Jersey, New Brunswick, New Jersey 08903 USA |
| |
Abstract: | The plasma membrane-bound penicillinase of has been purified. Amino acid analysis showed no significant differences in composition between the enzyme and exopenicillinase. Enzyme purified from cultures containing H333PO4 or 3H]-glycerol contained 33P or 3H]-glycerol activity and treatment with 8 M urea, 0.2% sodium dodecyl sulfate at 80° C did not remove the 3H-activity from the enzyme protein. Trypsin readily cleaved the glycerol-containing moiety from the enzyme protein, forming enzyme with molecular weight and heat stability like that of the exoenzyme. Phospholipase D and C also produced enzyme resembling the exo-form. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|