Continuous fractionation of proteins and mucopolysaccharides by simultaneous two-dimensional gel filtration and electrophoresis |
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| Authors: | Carl P. Dietrich |
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| Affiliation: | Departamento de Bioquímica e Farmacologia, Escola Paulista de Medicina, Caixa Postal 20372, 01000 São Paulo, SP - Brazil |
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| Abstract: | A method for continuous-loading Sephadex gel electrophoresis is described. Albumin, α1, α2, β and γ globulins, and two kininases and an amino-peptidase are completely separated and obtained at the preparative scale by this procedure. Heparan sulfates and abnormal mucopolysaccharides from the urine of patients with Hurler's syndrome were also fractionated by this method. |
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