盐酸沙拉沙星在鲫体内的残留及消除规律研究

采用高效液相色谱法测定鲫组织中沙拉沙星并初步研究了盐酸沙拉沙星在鲫组织中的残留及消除规律。在21±2℃下,以20mg/kg的剂量单次口灌给药,取血浆和肌肉、皮肤、肝胰脏、肾脏、卵巢5种组织,各样品中加入甲磺酸达氟沙星作内标,用二氯甲烷提取组织中的药物,正己烷去脂,反相高效液相色谱法测定其中盐酸沙拉沙星的浓度。此方法平均回收率均大于82.97%,日间变异系数小于8.41%,最低检测限可达0.0125μg/g。研究结果表明盐酸沙拉沙星在血浆和5种组织中消除速率快慢不一,肾脏为盐酸沙拉沙星残留的靶组织。若规定可食用组织中的盐酸沙拉沙星在最大残留限量为30μg/kg,由休药期(WDT)公式可得出盐酸沙拉沙星在鲫体内的WDT为14d。  

Studies on residue and depletion of sarafloxacin hydrochloride in Goldfish, Carassius auratus

This study developed a method to detect sarafloxacin in goldfish (Carassius auratus) plasma and tissues using reverse phase high performance liquid chromatography (RP2HPLC).Plasma,muscle,skin,hepatopancreas,kidney and ovary were sampled at different intervals after oral administration of sarafloxacin hydrochloride at single dose of 20mg/kg under 21±2 ℃.Joining danofloxacin mesylate as inner standard , the medicine was extracted by dichloromethane from plasma and tissues,and the extracts were evaporated to dryness with N2 flow in water bath of 60℃then dissolved in mobile phase . The fat of the solute was degreased by hexane.Sarafloxacin hydrochloride and danofloxacin mesylate analyses were performed by RP2HPLC.The mobile phase was acetonitrile and 0.12mol/L phosphate buffer (pH714) (including 015 % tetrabutyl ammonium bromide)(10/90,V/ V) adjusted to pH 310 with phosphoric acid.Sarafloxacin hydrochloride and danofloxacin mesylate were quantitated by using ZORAX SB2C18 column and ultraviolet detector was set at 280nm.The mean recoveries were all higher than 82.97% for samples and inter-day CV for plasma and tissues were all under 8.41%,The lowest limit of detection was 010125μg/g for muscle.Both limit of detection (LOD) and limit of quantitation (LOQ) met the requirement of the residual detection.The results showed that the elimination rate was markedly different in plasma and five tissues of healthy female goldfish. The drug was slowly eliminated in plasma,the concentration of sarafloxacin hydrochloride in kidney and hepatopancreas was higher than other tissues and skin showed the slowest depletion among five tissues.Kidney was the main reservoir of sarafloxacin hy- drochloride in goldfish.Withdrawl time was 14d when the maximum residue is 30μg/kg for edible tissues after single oral administration of sarafloxacin hydrochloride in healthy female goldfish.