Immunoaffinity-purified opiate receptor specifically binds the delta-class opiate receptor ligand, cis-(+)-3-methylfentanylisothiocyanate, SUPERFIT |
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| Authors: | D J Carr B DeCosta A E Jacobson K L Bost K C Rice J E Blalock |
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| Institution: | University of Alabama at Birmingham, Department of Physiology and Biophysics 35294. |
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| Abstract: | Using an antibody generated against the opiate receptor on NG108-15 cells, we recently purified the putative receptor from this hybrid cell line. We herein report that the purified receptor complex specifically binds tritiated cis-(+)-3-methylfentanylisothiocyanate (SUPERFIT), with the predominant binding associated with a 58 kDa polypeptide chain. Consistent with these findings is the in situ labeling of a 58 kDa protein with 3H]SUPERFIT on NG108-15 cells. |
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