In situ nick translation distinguishes between C-band positive regions on mouse chromosomes |
| |
Authors: | Sabine Adolph |
| |
Institution: | (1) Abteilung Klinische Genetik der Universität Ulm, Oberer Eselsberg M 25, D-7900 Ulm/Donau, Germany |
| |
Abstract: | In situ nick translation of mouse metaphase chromosomes by non-radioactive detection means and DNase I digestion followed by Giemsa staining were used to analyse the DNase I resistance of two different C-band positive regions. These were the centromeric heterochromatin of aero- and metacentric chromosomes and an interstitial C- band on chromosome 1 of wild mice, IS(HSR;1C5D)1Lub. Whereas the centromeric heterochromatin was clearly resistant to DNase I, the interstitial C-band showed very high DNase I sensitivity. Among centromeric C-bands, the heterochromatin in Robertsonian fusion biarmed chromosomes was more resistant to DNase I action than was the centromeric heterochromatin of the acrocentric chromosomes. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|