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Ethidium monoazide and propidium monoazide for elimination of unspecific DNA background in quantitative universal real-time PCR |
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| Authors: | Hein Ingeborg Schneeweiss Wilfried Stanek Christian Wagner Martin |
| Affiliation: | Institute of Milk Hygiene, Milk Technology and Food Science, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria. ingeborg.hein@vu-wien.ac.at |
| Abstract: | Unspecific background DNA in quantitative universal real-time PCR utilizing a hydrolysis probe was completely suppressed by the addition of EMA or PMA to the PCR mix via cross-linking of the dyes to DNA during 650 W visible light exposure. The proposed procedure had no effect on the sensitivity of the real-time PCR reaction. |
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