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Expression of Staphylococcus aureus enterotoxin type D in Escherichia coli X1776 |
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| Authors: | Robert C. Brown Alistair H.A. Bingham |
| Affiliation: | PHLS Centre for Applied Microbiology and Research, Porton, Salisbury, Wiltshire, U.K. |
| Abstract: | The Staphylococcus aureus enterotoxin type D was cloned and expressed in Escherichia coli X1776 either as mature toxin or as a fusion with E. coli beta-galactosidase. Regulated expression was obtained and levels of toxin produced were in the order of 10(-3)-fold higher than in S. aureus. |
| Keywords: | Staphyloccocal enterotoxin D Expression Escherichia coli Polymerase chain reaction β-Galactosidase fusion |