Inhibition of the nitrate reductase complex from spinach by oxylamines |
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Authors: | Narendra Jawali Prafullachandra Vishnu Sane |
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Institution: | Biology and Agriculture Division Bhabha Atomic Research Centre, Bombay 400085, India |
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Abstract: | The nitrate reductase complex from spinach (Spinacia oleracea) was found to be inhibited by oxylamine compounds such as aminooxyacetate, hydroxylamine and O-methoxylamine. These compounds appear to interact with reduced cytochrome b557 during catalysis of the enzyme. However, if the enzyme is maintained in a reduced state by NADH in the absence of nitrate, an additional component involved in FMNH2-nitrate reductase is also affected by them. The binding of the oxylamines with the enzyme is non-covalent in nature as the inhibition can be reversed by treatment with 2-oxoglutarate. |
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Keywords: | Chenopodiaceae spinach nitrate reductase complex oxylamines inhibition |
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