The effect of Fe-EDDHA and of ascorbic acid on in vitro rooting of the peach rootstock GF-677 explants |
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| Authors: | Chrysovalantou Antonopoulou Kortessa Dimassi Ioannis Therios Christos Chatzissavvidis Ioannis Papadakis |
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| Institution: | (1) Department of Horticulture, School of Agriculture, Aristotle University, 541 24 Thessaloniki, Greece;(2) Department of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, Greece |
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| Abstract: | The objective of this research was to study the effect of the chelated form of the iron salt of ethylenediamine di-o-hydroxyphenylacetic acid (Fe-EDDHA) (6% Fe) on in vitro rooting of the rootstock GF-677. The iron salt of ethylenediamine
tetraacetic acid (Fe-EDTA) (12% Fe) of the MS basic medium was replaced by Fe-EDDHA, which was applied in three concentrations:
93.5, 187.0 and 280 mg l−1 (5.6, 11.2 and 16.8 mg l−1 Fe, respectively). For each treatment of Fe-EDDHA, the effect of ascorbic acid added in four concentrations (0, 0.1, 1.0
and 10 mg l−1) was studied. After 4 weeks of culture, the explants growing on the medium with 280 mg l−1 Fe-EDDHA gave the best rooting results. Regarding ascorbic acid, no clear stimulating effect on rooting was found. |
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| Keywords: | Antioxidant Hybrid Iron Micropropagation Prunus Vitamin C |
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