Detection of a novel HLA-DQ specificity: serological and immunochemical analyses by a monoclonal antibody |
| |
| Authors: | Naoshi Ishikawa Hiroshi Kojima Tsuguyo Nakayama Hiroshi Kunikane Shuichi Hawkin Yu-ichiro Fukasawa Hitoshi Ikeda Kazumasa Ogasawara Masanori Kasahara Yasutaka Tajima Yukio Kakuta Akemi Wakisaka Miki Aizawa |
| |
| Affiliation: | (1) Department of Pathology, Hokkaido University School of Medicine, 060 Sapporo, Japan;(2) Present address: Division of Molecular Pathology and Genetics, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, 173 Itabashi-ku, Tokyo, Japan;(3) Present address: Institute of Immunological Science, Hokkaido University, Sapporo, Japan;(4) Present address: Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida, USA |
| |
| Abstract: | ![]()
A monoclonal antibody (mAb) with a novel human B-cell allospecificity was produced by immunizing a C3H/He mouse with the human B lymphoblastoid cell line EBV-Wa (HLA-DR4/Dw15/DQblank homozygous). The mAb, termed HU-46, reacted with B cells from not only DR4/Dw15-positive individuals but also certain DRw8/Dw8-positive ones whose DQ phenotypes had not yet been defined. Two-dimensional gel analyses indicated that the mAb recognized class II antigens which were encoded by the HLA-DQ locus. Furthermore, in genetic analysis, the gene encoding the class II antigen detected by HU-46 met the Hardy-Weinberg condition as a fourth allele of the DQ locus. We provisionally labeled this novel DQ specificity DQWa. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
