由小-簇麦杂种悬浮细胞和原生质体再生植株

１９８８年以来，小麦原生质体培养取得了重要进展［１—７］，但成功还仅限于少数基因型，因此，为了建立和不断完善小麦及其他禾谷类植物原生质体培养的技术体系，还有待在更多的基因型中进行探索。在小麦远缘杂种系统中，１９９０年王铁邦等［８］培养小－偃麦原生质体获得成功。本文报道由普通小麦－簇毛麦杂种悬浮细胞和原生质体再生植株的结果。材料和方法起始材料取自本实验室继代保存近２年的小－簇麦杂种（２ｎ＝４ｘ＝２８）愈伤组织。该愈伤组织是由小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．，品种：“农大１４６”）×簇毛麦…

PLANT REGENERATION FROM CELL AND PROTOPLAST CULTURES OF TRITICUM AESTIVUM HAYNALDIA VILLOSAHYBRID

Starting materials used in these experiments were taken from Triticum aestivum Haynaldia villosa hybrid embryo derived callus, which had been maintained for nearly two years. To establish suspension cultures, the callus was subcultured till its compact texture became friable, and then shaken in a liquid medium. Upon transferring the suspended cells onto a semisolid medium, high frequency of plant regeneration was achieved.